Authors
Corry-Anke Brandsma, Maarten van den Berge, Dirkje S Postma, Marnix R Jonker, Sharon Brouwer, Peter D Paré, Don D Sin, Yohan Bossé, Michel Laviolette, Juha Karjalainen, Rudolf SN Fehrmann, David C Nickle, Ke Hao, Anita IR Spanjer, Wim Timens, Lude Franke
Publication date
2015/1/1
Journal
Thorax
Volume
70
Issue
1
Pages
21-32
Publisher
BMJ Publishing Group Ltd
Description
Background
Chronic obstructive pulmonary disease (COPD) is a progressive, incurable lung disease characterised by abnormal tissue repair causing emphysema and small airways fibrosis. Since current therapy cannot modify this abnormal repair, it is crucial to unravel its underlying molecular mechanisms. Unbiased analysis of genome-wide gene expression profiles in lung tissue provides a powerful tool to investigate this.
Methods
We performed genome-wide gene expression profiling in 581 lung tissue samples from current and ex-smokers with (n=311) and without COPD (n=270). Subsequently, quantitative PCR, western blot and immunohistochemical analyses were performed to validate our main findings.
Results
112 genes were found to be upregulated in patients with COPD compared with controls, whereas 61 genes were downregulated. Among the most upregulated genes were fibulin-5 (FBLN5 …
Scholar articles
CA Brandsma, M van den Berge, DS Postma… - Thorax, 2015