Authors
Julie E Lang, Alexander Ring, Tania Porras, Pushpinder Kaur, Victoria A Forte, Neal Mineyev, Debu Tripathy, Michael F Press, Daniel Campo
Publication date
2018/8
Journal
Annals of surgical oncology
Volume
25
Pages
2261-2270
Publisher
Springer International Publishing
Description
Background
We characterized the whole transcriptome of circulating tumor cells (CTCs) in stage II–III breast cancer to evaluate correlations with primary tumor biology.
Methods
CTCs were isolated from peripheral blood (PB) via immunomagnetic enrichment followed by fluorescence-activated cell sorting (IE/FACS). CTCs, PB, and fresh tumors were profiled using RNA-seq. Formalin-fixed, paraffin-embedded (FFPE) tumors were subjected to RNA-seq and NanoString PAM50 assays with risk of recurrence (ROR) scores.
Results
CTCs were detected in 29/33 (88%) patients. We selected 21 cases to attempt RNA-seq (median number of CTCs = 9). Sixteen CTC samples yielded results that passed quality-control metrics, and these samples had a median of 4,311,255 uniquely mapped reads (less than PB or tumors …
Scholar articles
JE Lang, A Ring, T Porras, P Kaur, VA Forte, N Mineyev… - Annals of surgical oncology, 2018