Authors
Chad A Ray, Ronald R Bowsher, Wendell C Smith, Viswanath Devanarayan, Mark B Willey, John T Brandt, Robert A Dean
Publication date
2005/1/4
Journal
Journal of pharmaceutical and biomedical analysis
Volume
36
Issue
5
Pages
1037-1044
Publisher
Elsevier
Description
Quantification of biomarkers can provide important information about the safety and efficacy of candidate drugs. Unfortunately, limited sample volume and excess costs often limit analysis of multiple biomarkers. We developed, optimized, validated, and implemented a multiplex immunoassay for simultaneous measurement of multiple circulating cytokines: IL-1β, TNFα, IL-6, IL-8, and IL-10. Multiplex immuoassays were performed using the Luminex LabMAP instrument. Capture antibodies for each cytokine were covalently bound to distinct microsphere subsets distinguished by differing dye ratios. The concentration of each individual cytokine determined by measuring orange fluorescence produced by a complex of a biotinylated cytokine-specific antibody and streptavidin-phycoerythrin. The lower limit of quantification for all assays was 20pg/mL with the exception of IL-8 which was 100pg/mL. The inter-assay …
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Scholar articles
CA Ray, RR Bowsher, WC Smith, V Devanarayan… - Journal of pharmaceutical and biomedical analysis, 2005