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The aromatic amines paru-tyramine (p-TYM) and paru-octopamine(p-OCT) may serve as neuromodulators and neurotransmitters in mammalian tissues (l-6). These amines and the enzymes that synthesize them are widely distributed in mammalian tissues (3, 7, 8) and are present in many invertebrates (9, lO). They have also been implicated in the etiology of a variety of pathological states, including depression, schizophrenia, Parkinsonism, migraine, and hypertension (11-15). Several analytical methods have been developed for the quantification of p-TYM and p-OCT in tissues and biologic fluids (16-22). Some of them have low sensitivity, particularly in plasma, and others require mass spectroscopy, gas chromatography, or high-performance liquid chromatography (HPLC). Additionally, some earlier assays lack necessary specificity since they cannot distinguish between the various positional isomers of TYM and …