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Landscape fragmentation, i.e. the process where large habitat patches become smaller and more isolated, has often been accelerated by human activities, such as deforestation, agricultural land conversion, and urbanisation of natural areas. Transport and mobility infrastructures are a major cause of landscape fragmentation. The Infrastructural Fragmentation Index is a common measure of landscape fragmentation due to transport and mobility infrastructures and depends, inter alia, on the occlusion coefficient, which accounts for the obstruction to movement. The values of this coefficient mirror well-established conditions, which depend on type of transport and mobility infrastructure and traffic flow. Lack of data affects its values and generates uncertainty in the measurement of landscape fragmentation. In this study, we develop on a sensitivity analysis, by assessing how IFI varies when the occlusion coefficient …