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Tropospheric ozone (O₃) is a phytotoxic air pollutant causing a substantial damage to plants and agriculture worldwide. Plant productivity is affected by several environmental factors, which interact with each other. Studies related to interactions involving O₃ and different levels of nitrogen (N) are still rare and elusive. In the present study we grew two wheat cultivars (HD2967 and Sonalika) in open top chambers (OTC) under ambient (AO) and elevated O₃ (EO) (ambient + 20 ppb O₃) and provided two levels of N fertilization; (a) recommended nitrogen (RN), (b) 1.5 times the recommended N (HN). Growth (root/shoot ratio, leaf number and leaf area), biomass, gas-exchange (stomatal conductance (g_s), photosynthesis (A), transpiration (E), chlorophyll fluorescence (F_v/F_m), physiological (chlorophyll and carotenoids), biochemical [antioxidant activity, lipid peroxidation (MDA)] parameters and leaf N content were …