Authors
Robert S Janssen, Glen A Satten, Susan L Stramer, Bhupat D Rawal, Thomas R O'Brien, Barbara J Weiblen, Frederick M Hecht, Noreen Jack, Farley R Cleghorn, James O Kahn, Margaret A Chesney, Michael P Busch
Publication date
1998/7/1
Journal
Jama
Volume
280
Issue
1
Pages
42-48
Publisher
American Medical Association
Description
Context
Differentiating individuals with early human immunodeficiency virus 1 (HIV-1) infection from those infected for longer periods is difficult but important for estimating HIV incidence and for purposes of clinical care and prevention.
Objective
To develop and validate a serologic testing algorithm in which HIV-1–positive persons with reactive test results on a sensitive HIV-1 enzyme immunoassay (EIA) but nonreactive results on a less sensitive (LS) EIA are identified as having early infection.
Design
Diagnostic test and testing strategy development, validation, and application. Specimens were tested with both a sensitive HIV-1 EIA (3A11 assay) and a less sensitive modification of the same EIA (3A11-LS assay).
Settings and Participants
For assay development: 104 persons seroconverting to HIV-1 comprising 38 plasma donors, 18 patients of a sexually transmitted disease clinic in Trinidad, and 48 participants in the …
Total citations
Scholar articles
RS Janssen, GA Satten, SL Stramer, BD Rawal… - Jama, 1998