Authors
José F Siqueira Jr, Isabela N Rôças, Renata Souto, Milton de Uzeda, Ana Paula Colombo
Publication date
2002/3/1
Journal
Journal of endodontics
Volume
28
Issue
3
Pages
168-172
Publisher
Elsevier
Description
The purpose of this study was to evaluate the prevalence of Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections by using a molecular genetic method. Samples were obtained from 53 infected teeth, of which 27 cases were diagnosed as acute periradicular abscesses. DNA was extracted to evaluate the occurrence of 13 bacterial species by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. Polymerase chain reaction using an ubiquitous bacterial primer was undertaken to check the presence of bacterial DNA in clinical samples. All root canal samples contained bacteria as demonstrated by polymerase chain reaction. The checkerboard DNA-DNA hybridization assay allowed the detection of streptococci in 22.6% of the samples, Actinomyces species in 9.4%, and E. faecalis in 7.5%. The most prevalent species were members of the Streptococcus …
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Scholar articles
JF Siqueira Jr, IN Rôças, R Souto, M de Uzeda… - Journal of endodontics, 2002