Authors
José F Siqueira Jr, Isabela N Rôças, Renata Souto, Milton de Uzeda, Ana Paula Colombo
Publication date
2000/6/1
Journal
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
Volume
89
Issue
6
Pages
744-748
Publisher
Mosby
Description
OBJECTIVE
The purpose of this investigation was to examine the microbiota of infected root canals by using a molecular genetic method.
STUDY DESIGN
The presence and levels of 42 bacterial species were determined in 28 root canal samples by using whole genomic DNA probes and checkerboard DNA-DNA hybridization. To confirm the presence of bacterial DNA in clinical samples, a polymerase chain reaction with an ubiquitous bacterial primer was undertaken.
RESULTS
The results of the checkerboard DNA-DNA hybridization analysis showed that 22 of the 42 DNA probes tested were reactive with 1 or more samples. The number of bacterial species in the root canal samples ranged from 1 to 17 (mean, 4.7). Seventeen of the 28 root canal samples were positive for at least 1 DNA probe. The most prevalent species found were as follows: Bacteroides forsythus (39.3% of the cases); Haemophilus aphrophilus …
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Scholar articles
JF Siqueira Jr, IN Rôças, R Souto, M de Uzeda… - Oral Surgery, Oral Medicine, Oral Pathology, Oral …, 2000