Authors
Anuj Rana, Baljeet Singh Saharan, Monica Joshi, Radha Prasanna, Kanika Kumar, Lata Nain
Publication date
2011/12
Journal
Annals of microbiology
Volume
61
Issue
4
Pages
893-900
Publisher
BioMed Central
Description
Development of an effective plant growth promoting rhizobacteria (PGPR) inoculant necessitates the presence of a diverse set of traits that can help its colonization of the rhizosphere and survival under varying environmental conditions. In our investigation, a set of 100 bacterial isolates from the rhizosphere of wheat plants were screened initially on the basis of a seed germination assay; ten bacterial isolates (AW1–AW10) were selected. These isolates were then tested in vitro for specific PGPR traits, such as the production of IAA, siderophore, ammonia, HCN, P solubilization, ACC deaminase activity, acetylene reduction assay and antifungal activity. Of the ten isolates, AW5 was found to be promising for all PGP attributes. An experiment undertaken in the controlled conditions of the National Phytotron Facility revealed the potential of three isolates (AW1, AW5 and AW7) in enhancing the growth parameters …
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