Authors
Philippe Corbisier, Daniel Van Der Lelie, Brigitte Borremans, Ann Provoost, Victor De Lorenzo, Nigel L Brown, Jonathan R Lloyd, Jonathan L Hobman, Elisabeth Csöregi, Gillis Johansson, Bo Mattiasson
Publication date
1999/4/28
Journal
Analytica chimica acta
Volume
387
Issue
3
Pages
235-244
Publisher
Elsevier
Description
The principal goal of this work was to establish the feasibility of two biosensor technologies with enhanced specificity and selectivity for the detection of several bioavailable heavy metals in environmental samples. Two parallel strategies have been followed. The first approach was to construct whole cell bacterial biosensors that emit a bioluminescent or fluorescent signal in the presence of a biologically available heavy metal. The molecular basis of σ-54 promoters as sensing elements of environmental pollutants has been determined and a number of metal-induced promoter regions have been identified, sequenced and cloned as promoter cassettes. The specificity of the promoter cassettes has been determined using luxCDABE reporter systems. Whole cell-biosensors containing metal-induced lux reporter systems have been incorporated into different matrices for their later immobilisation on optic fibres and …
Total citations
Scholar articles
P Corbisier, D Van Der Lelie, B Borremans, A Provoost… - Analytica chimica acta, 1999