Authors
Jin Zhang, Malachi Griffith, Christopher A Miller, Obi L Griffith, David H Spencer, Jason R Walker, Vincent Magrini, Sean D McGrath, Amy Ly, Nichole M Helton, Maria Trissal, Daniel C Link, Ha X Dang, David E Larson, Shashikant Kulkarni, Matthew G Cordes, Catrina C Fronick, Robert S Fulton, Jeffery M Klco, Elaine R Mardis, Timothy J Ley, Richard K Wilson, Christopher A Maher
Publication date
2017/11/1
Journal
Experimental Hematology
Volume
55
Pages
19-33
Publisher
Elsevier
Description
Highlights
- Current sequencing strategies provide inadequate characterization of the diversity of RNAs.
- Deep sequencing on 17–100 nt revealed previously unannotated small RNAs.
- Novel lncRNAs were discovered and validated from ultra-deep transcriptome sequencing.
To detect diverse and novel RNA species comprehensively, we compared deep small RNA and RNA sequencing (RNA-seq) methods applied to a primary acute myeloid leukemia (AML) sample. We were able to discover previously unannotated small RNAs using deep sequencing of a library method using broader insert size selection. We analyzed the long noncoding RNA (lncRNA) landscape in AML by comparing deep sequencing from multiple RNA-seq library construction methods for the sample that we studied and then integrating RNA-seq data from 179 AML cases. This identified lncRNAs that are completely novel, differentially expressed …
Scholar articles
J Zhang, M Griffith, CA Miller, OL Griffith, DH Spencer… - Experimental hematology, 2017