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Most insular communities exhibit nestedness, with the species assemblages of the more depauperate islands constituting subsets of those of the richer. Several methods for the estimation and evaluation of nestedness have been developed during the last fifteen years. In this paper we use two of the more recent and elaborate methods, namely the ‘temperature’ method of Atmar and Patterson and the ‘departures’ method of Lomolino, in order to investigate patterns of nestedness in the distribution of two well studied and speciose animal groups, terrestrial isopods and land snails, in the Kyklades archipelago (Aegean Sea. Greece) that lies between two continental regions, Significant nestedness is present in both species assemblages and, surprisingly, each method gives almost identical levels of nestedness for the two animal groups. Isolation has been found to be more important in producing nestedness in both …