Authors
Noel Britton, Haopu Yang, Adam Fitch, Kelvin Li, Khaled Seyed, Rui Guo, Shulin Qin, Yingze Zhang, William Bain, Faraaz Shah, Partha Biswas, Wonseok Choi, Malcolm Finkelman, Yonglong Zhang, Catherine L Haggerty, Panayiotis V Benos, Maria M Brooks, Bryan J McVerry, Barbara Methe, Georgios D Kitsios, Alison Morris
Publication date
2023/5/16
Journal
medRxiv
Publisher
Cold Spring Harbor Laboratory Preprints
Description
Objectives
We investigated whether mycobiota variation in the respiratory tract is associated with host-response and clinical outcomes in critically ill patients.
Methods
To characterize the upper and lower respiratory tract mycobiota, we performed rRNA gene sequencing (internal transcribed spacer) of oral swabs and endotracheal aspirates (ETA) from 316 mechanically-ventilated patients. We examined associations of mycobiome profiles (diversity and composition) with clinical variables, host-response biomarkers, and outcomes.
Measurements and Main Results
ETA samples with> 50% relative abundance for C. albicans (51%) were associated with elevated plasma IL-8 and pentraxin-3 (p= 0.05), longer time-to-liberation from mechanical ventilation (p= 0.04) and worse 30-day survival (adjusted hazards ratio (adjHR): 1.96 [1.04–3.81], p= 0.05). Using unsupervised clustering, we derived two clusters in ETA samples …
Total citations
Scholar articles
N Britton, H Yang, A Fitch, K Li, K Seyed, R Guo, S Qin… - medRxiv, 2023