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Figure S1 Histograms of total nuclear DAPI intensities and fitted cell cycle phase curves. Total DAPI intensities per nucleus were calculated for SKBR3 cells and plotted as probability density histograms at four different trastuzumab concentrations. A prominent peak for G1 phase was observed, a plateau representing S phase and a smaller peak at approximately two times the DNA content of G1 phase cells, consisting of cells in G2/M phase. Solid line graphs represent either the G1 phase term (red), S phase term (blue) or G2/M phase term (green) of the resulting fit (cyan). Integration over the individual terms yielded relative quantities for each cell cycle phase (see Figure 2).