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Hydrocarbons and their derivative compounds are recalcitrant in nature and causing adverse impacts to the environment and are classified as important pollutants. Removal of these pollutants from the atmosphere is a challenging process. Hydrophobic organic pollutants (HOPs) including crude oil, diesel, dotriacontane (C₃₂), and tetracontane (C₄₀) are subjected to the biodegradation study by using a bacterial consortium consist of Bacillus subtilis, Pseudomonas stutzeri, and Acinetobacter baumannii. The impact of pH and temperature on the biodegradation process was monitored. During the HOPs biodegradation, the impact of hydrocarbon-degrading extracellular enzymes such as alcohol dehydrogenase, alkane hydroxylase, and lipase was examined, and found average activity about 47.2, 44.3, and 51.8 μmol/mg⁻¹, respectively. Additionally, other enzymes such as catechol 1,2 dioxygenase and catechol 2,3 …