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Presented here is a late Quaternary pollen record from Laguna La Gaiba (17 45S, 57 35W), a 55 km2 lake located at the western margin of the Pantanal basin, the world's largest tropical wetland, and the eastern limit of the Bolivian Chiquitano dry forest. A suite of 12 AMS radiocarbon dates on terrestrial macrofossils demonstrates a continuous sediment record spanning at least the last 60,000 years. Today, upland areas of the lake catchment are blanketed by closed-canopy semi-deciduous forest. However, arboreal pollen was largely absent from glacial-age sediments, indicative of a climate drier than present, and a landscape dominated by open, herbaceous savanna. Tropical forest appeared during the glacial-Holocene transition, pointing to increased precipitation, but was floristically different from those of the Holocene. Seasonally-dry tropical forest, floristically similar to that of today, appeared during the early …