Authors
Ferda Cevikbas, Xidao Wang, Tasuku Akiyama, Cordula Kempkes, Terhi Savinko, Attila Antal, Gabriela Kukova, Timo Buhl, Akihiko Ikoma, Joerg Buddenkotte, Vassili Soumelis, Micha Feld, Harri Alenius, Stacey R Dillon, Earl Carstens, Bernhard Homey, Allan Basbaum, Martin Steinhoff
Publication date
2014/2/1
Journal
Journal of allergy and clinical immunology
Volume
133
Issue
2
Pages
448-460. e7
Publisher
Mosby
Description
Background
Although the cytokine IL-31 has been implicated in inflammatory and lymphoma-associated itch, the cellular basis for its pruritic action is yet unclear.
Objective
We sought to determine whether immune cell–derived IL-31 directly stimulates sensory neurons and to identify the molecular basis of IL-31–induced itch.
Methods
We used immunohistochemistry and quantitative real-time PCR to determine IL-31 expression levels in mice and human subjects. Immunohistochemistry, immunofluorescence, quantitative real-time PCR, in vivo pharmacology, Western blotting, single-cell calcium imaging, and electrophysiology were used to examine the distribution, functionality, and cellular basis of the neuronal IL-31 receptor α in mice and human subjects.
Results
Among all immune and resident skin cells examined, IL-31 was predominantly produced by TH2 and, to a significantly lesser extent, mature dendritic cells …
Scholar articles
F Cevikbas, X Wang, T Akiyama, C Kempkes… - Journal of allergy and clinical immunology, 2014