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The Michelson Interferometer for Global High-resolution Thermospheric Imaging (MIGHTI) was launched aboard NASA’s Ionospheric Connection (ICON) Explorer satellite in October 2019 to measure winds and temperatures on the limb in the upper mesosphere and lower thermosphere (MLT). Temperatures are observed using the molecular oxygen atmospheric band near 763 nm from 90–127 km altitude in the daytime and 90–108 km in the nighttime. Here we describe the measurement approach and methodology of the temperature retrieval, including unique on-orbit operations that allow for a better understanding of the instrument response. The MIGHTI measurement approach for temperatures is distinguished by concurrent observations from two different sensors, allowing for two self-consistent temperature products. We compare the MIGHTI temperatures against existing MLT space-borne and ground-based …