Authors
Guohui Wu, Jaroslaw Majewski, Canay Ege, Kristian Kjaer, Markus Jan Weygand, Ka Yee C Lee
Publication date
2005/11/1
Journal
Biophysical journal
Volume
89
Issue
5
Pages
3159-3173
Publisher
Elsevier
Description
The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.
Total citations
Scholar articles
G Wu, J Majewski, C Ege, K Kjaer, MJ Weygand… - Biophysical journal, 2005