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Model estimation and structure detection with short data records are two issues that receive increasing interests in System Identification. In this paper, a multiple kernel-based regularization method is proposed to handle those issues. Multiple kernels are conic combinations of fixed kernels suitable for impulse response estimation, and equip the kernel-based regularization method with three features. First, multiple kernels can better capture complicated dynamics than single kernels. Second, the estimation of their weights by maximizing the marginal likelihood favors sparse optimal weights, which enables this method to tackle various structure detection problems, e.g., the sparse dynamic network identification and the segmentation of linear systems. Third, the marginal likelihood maximization problem is a difference of convex programming problem. It is thus possible to find a locally optimal solution efficiently by …