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Little attention has been paid so far to physiological signals for emotion recognition compared to audiovisual emotion channels such as facial expression or speech. This paper investigates the potential of physiological signals as reliable channels for emotion recognition. All essential stages of an automatic recognition system are discussed, from the recording of a physiological data set to a feature-based multiclass classification. In order to collect a physiological data set from multiple subjects over many weeks, we used a musical induction method that spontaneously leads subjects to real emotional states, without any deliberate laboratory setting. Four-channel biosensors were used to measure electromyogram, electrocardiogram, skin conductivity, and respiration changes. A wide range of physiological features from various analysis domains, including time/frequency, entropy, geometric analysis, subband spectra …