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Plasmonic biosensing is an optical technique that based on refractive index change when the target molecules interact with the sensing surface. Main plasmonic material used in this type of biosensors is gold. Our work is dedicated to design a novel sensing SPR chip with vanadium dioxide (VO2) nanolayer, known for its unique insulator-to-metal phase transition in the near-infrared region. VO2 thin film is deposited using Cross-Beam Pulsed Laser Deposition (CB-PLD) method and gold layer deposition is performed by sputtering. By employing the VO2 nanolayer, we create a highly responsive biosensing interface (with a much-improved sensitivity and also a wide dynamic measurement range). The VO2 layer's ability to modulate the refractive index enables precise control of the excited plasmon resonance. This interaction results in enhancing sensitivity and the capability to detect low-concentration analytes with …