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Our aim was to evaluate the role of Th17 and its balance with Treg cells in defining CHIK chronification pathogenesis, as well as its association with clinical scores. We hypothesized that given that Chikungunya (CHIK) is caused by an arthritogenic alphavirus and possess several similarities with rheumatoid arthritis (RA), both characterized by Th17 cells playing a pathogenic role, in association with limited studies on the role of T cells regarding this CHIK chronification process. To reach this aim, without suitable animal models of CHIKV to properly assess the development of disease clinical signs, we developed a model of CHIK in DBA1/J mice associated with collagen-induced arthritis (CIA). Virus titration was obtained through One Step qPCR. Flow cytometry analyses were performed through cells isolation of draining lymph nodes from CHIKV previously infected (n= 6), CIA (n= 6) and CHIKV+ CIA (n= 7). As for …