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The aim of this work was to characterize T regulatory cells (Treg) from patients presenting both acute and chronic forms of Chikungunya disease. To reach this aim, peripheral blood samples from controls (n= 7), acute (n= 7) and chronic (n= 6) patients were collected and evaluated by flow cytometry regarding Treg markers such as CD39, CD73, Granzyme B, Perforin, PD-1, CTLA-4, INF-γ, IL-10 and TGF-β. It was observed: 1) Decrease in Treg cells frequency of acute and chronic patients compared to controls; 2) Decreased expression of extracellular ATP cleavage enzymes (CD39/CD73) in Treg cells from both patients groups; 3) Treg cells from acute and chronic patients presented a decreased production of Granzyme B and Perforin compared to control group; 4) Treg cells of acute patients showed a decrease of 50% in CTLA-4 expression, which is reestablished in patients presenting the chronic form of the …