Authors
Bruno Rafael Virginio de Sousa, Alexandre Sérgio Silva, Caroline Severo de Assis, Tainá Gomes Diniz, Marina Gonçalves Monteiro Viturino, Isabella Wanderley de Queiroga Evangelista, Luiz Henrique Agra Cavalcante-Silva, Tatjana Souza Lima Keesen, Naila Francis Paulo de Oliveira, Darlene Camati Persuhn
Publication date
2024/4/18
Journal
Journal of Diabetes & Metabolic Disorders
Pages
1-10
Publisher
Springer International Publishing
Description
Purpose
To investigate the potential relation between methylation of miR-9-3 and stages of diabetic retinopathy (DR). Additionally, we explored whether miR-9-3 methylation impacts the serum levels of Vascular Endothelial Growth Factor (VEGF).
Methods
A cross-sectional study was conducted with 170 participants with type 2 diabetes, including a control group (n = 64) and a diabetes retinopathy group (n = 106), which was further divided into NPDR (n = 58) and PDR (n = 48) subgroups. Epidemiological, clinical, anthropometric, biochemical ELISA assay were analysed. DNA extracted from leukocytes was used to profile miR-9-3 methylation using PCR-MSP.
Results
MiR-9-3 hypermethylated profile was higher in the DR group (p < 0.001) and PDR subgroup compared to DM2 control group (p < 0.001). The hypermethylated profile in the PDR subgroup was also higher compared to NPDR subgroup (p < …
Scholar articles
BRV de Sousa, AS Silva, CS de Assis, TG Diniz… - Journal of Diabetes & Metabolic Disorders, 2024