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In this study, poultry litter was used as inoculums to increase biogas production from biomethanation of water hyacinth (WH) at mesophilic condition. A series of laboratory experiments using 250 ml biodigesters were performed in batch operation mode. 4 grams of completely dried and ground water hyacinth were fed to each biodigester and mixed with poultry litter inoculums (PLI) and water (W) in different ratios resulting in five different fermentation slurries (PLI-0, PLI-25, PLI-50, PLI-75 and PLI-100) with different total solids (TS) contents of 3.8, 5.5, 7.2, 8.9 and 10.6% respectively. Digester PL-100 fed with pure PLI without water hyacinth was considered as blank. The results showed that the PLI inoculated to biodigester significantly affect the biogas production. PLI influenced biogas production and the increase was more than two times when compared to water hyacinth substrate without PLI. The best performance for biogas production was from the digester PLI-75 followed by PLI-50 and PLI-100 whose TS contents are 8.9, 7.2 and 10.6% respectively. These results suggest that, TS content affects the biogas yield and optimum TS content for biogas production is between 7 and 9%.