Authors
Shams Uzzaman, Ayaz Mahmood Dar, Aamir Sohail, Sheraz Bhat, Yusuf Khan
Publication date
2014/1/3
Journal
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Volume
117
Pages
493-501
Publisher
Elsevier
Description
A series of new steroidal 4H-pyrans (4–6) have been synthesized from steroidal α, β-unsaturated ketones (1–3). The products (4–6) were characterized by IR, 1H NMR, 13C NMR, MS and analytical data. The interaction studies of compounds (4–6) with DNA were carried out by employing gel electrophoresis, UV–vis and fluorescence spectroscopy. The gel electrophoresis pattern revealed that compounds (4–6) bind to DNA and also demonstrated that the compound 6 alone or in presence of Cu (II) causes the nicking of supercoiled pBR322. The compounds 4 and 5 bind to DNA preferentially through electrostatic and hydrophobic interactions with Kb values found to be 5.3 × 103 and 3.7 × 103 M−1, respectively, indicating the higher binding affinity of compound 4 towards DNA. The docking study suggested the intercalation of compounds in between the nucleotide base pairs. The cytotoxicity and genotoxicity of the …
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Scholar articles
S Uzzaman, AM Dar, A Sohail, S Bhat, Y Khan - Spectrochimica Acta Part A: Molecular and …, 2014