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The present study sought to quantify the relationships among physical activity (PA), health-related fitness, and motor skill in children (grades 4 to 6), and to determine whether specific tests of fitness or motor skill are independently associated with objectively measured PA level. Four hundred and ninety-one students (56.4% female) wore a Digi-Walker pedometer for 7 consecutive days. Standardized protocols were used to assess health-related fitness (body mass index percentile, waist circumference, 20-m shuttle run, plank, handgrip, and trunk flexibility). Motor skill was evaluated using a validated obstacle course. Pearson correlations (with Holm adjustments for multiple comparisons) initially assessed associations among PA, health-related fitness, and motor skill. Multi-variable linear regression was used to determine which factors were significantly associated with daily step counts, while adjusting for gender …