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Characterization of preferential flow at multiple spatial and temporal scales is fundamental to the understanding of complex subsurface heterogeneity and catchment hydrology. Evidence of subsurface preferential flow and the conditions under which it occurs were investigated in the Shale Hills catchment, a humid forested region in central Pennsylvania, USA. Seven monitoring sites, plus five replicates, were established along a concave hillslope, a convex hillslope and a valley floor to monitor in situ the hydrology in various soil horizons and their interfaces at half‐minute intervals. Using the indicator of a lower horizon that responded to a rainstorm earlier than an upper horizon within the same soil profile, we investigated the subsurface preferential flow processes and their dynamics in each of the five soil series mapped in the catchment. Threshold behaviour, hydrophobicity impact, influence of soil thickness and …