Authors
Paolo Miotto, Belay Tessema, Elisa Tagliani, Leonid Chindelevitch, Angela M Starks, Claudia Emerson, Debra Hanna, Peter S Kim, Richard Liwski, Matteo Zignol, Christopher Gilpin, Stefan Niemann, Claudia M Denkinger, Joy Fleming, Robin M Warren, Derrick Crook, James Posey, Sebastien Gagneux, Sven Hoffner, Camilla Rodrigues, Iñaki Comas, David M Engelthaler, Megan Murray, David Alland, Leen Rigouts, Christoph Lange, Keertan Dheda, Rumina Hasan, Uma Devi K Ranganathan, Ruth McNerney, Matthew Ezewudo, Daniela M Cirillo, Marco Schito, Claudio U Köser, Timothy C Rodwell
Publication date
2017/12/1
Journal
European Respiratory Journal
Volume
50
Issue
6
Publisher
European Respiratory Society
Description
A clear understanding of the genetic basis of antibiotic resistance in Mycobacterium tuberculosis is required to accelerate the development of rapid drug susceptibility testing methods based on genetic sequence.
Raw genotype–phenotype correlation data were extracted as part of a comprehensive systematic review to develop a standardised analytical approach for interpreting resistance associated mutations for rifampicin, isoniazid, ofloxacin/levofloxacin, moxifloxacin, amikacin, kanamycin, capreomycin, streptomycin, ethionamide/prothionamide and pyrazinamide. Mutation frequencies in resistant and susceptible isolates were calculated, together with novel statistical measures to classify mutations as high, moderate, minimal or indeterminate confidence for predicting resistance.
We identified 286 confidence-graded mutations associated with resistance. Compared to phenotypic methods, sensitivity (95% CI) for …
Scholar articles
P Miotto, B Tessema, E Tagliani, L Chindelevitch… - European Respiratory Journal, 2017