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Vitamin‐A deficiency (VAD) is a major malnutrition problem in South Asia, where indica rice is the staple food. Indica‐type rice varieties feed more than 2 billion people. Hence, we introduced a combination of transgenes using the biolistic system of transformation enabling biosynthesis of provitamin A in the endosperm of several indica rice cultivars adapted to diverse ecosystems of different countries. The rice seed‐specific glutelin promoter (Gt‐1 P) was used to drive the expression of phytoene synthase (psy), while lycopene β‐cyclase (lcy) and phytoene desaturase (crtI), fused to the transit peptide sequence of the pea‐Rubisco small subunit, were driven by the constitutive cauliflower mosaic virus promoter (CaMV35S P). Transgenic plants were recovered through selection with either CaMV35S P driven hph (hygromycin phosphotransferase) gene or cestrum yellow leaf curling virus promoter (CMP) driven pmi …