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Seventy-eight mungbean genotypes were grown in the field and 28 genotypes were found to be resistant to Mungbean yellow mosaic virus, while the remainder were susceptible. All genotypes were further subjected to agroinoculation to confirm their resistance/susceptibility against MYMV using two different MYMV strains, namely VA 221 and VA 239. The results showed that only three genotypes, namely ML 1108, KMG 189 and SP 84, exhibited resistance to the VA 221 strain while 77 genotypes were found to be susceptible to strain VA 239. Only one genotype, namely ML 818, showed resistance to strain VA 239, but it was susceptible to strain VA 221. The accumulation of the viral genome in the host was confirmed by PCR amplification of the coat protein gene of MYMV using gene-specific primers.