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Accurately capturing medium- to low-frequency trends in tree-ring data is vital to assessing climatic response and developing robust reconstructions of past climate. Non-climatic disturbance can affect growth trends in tree-ring-width (RW) series and bias climate information obtained from such records. It is important to develop suitable strategies to ensure the development of chronologies that minimize these medium- to low-frequency biases. By performing high density sampling (760 trees) over a ~40-ha natural high-elevation Norway spruce (Picea abies) stand in the Romanian Carpathians, this study assessed the suitability of several sampling strategies for developing chronologies with an optimal climate signal for dendroclimatic purposes. There was a roughly equal probability for chronologies (40 samples each) to express a reasonable (r = 0.3–0.5) to non-existent climate signal. While showing a strong high …