Authors
Sadia Saeed, Jessica Quintin, Hindrik HD Kerstens, Nagesha A Rao, Ali Aghajanirefah, Filomena Matarese, Shih-Chin Cheng, Jacqueline Ratter, Kim Berentsen, Martijn A van der Ent, Nilofar Sharifi, Eva M Janssen-Megens, Menno Ter Huurne, Amit Mandoli, Tom van Schaik, Aylwin Ng, Frances Burden, Kate Downes, Mattia Frontini, Vinod Kumar, Evangelos J Giamarellos-Bourboulis, Willem H Ouwehand, Jos WM van der Meer, Leo AB Joosten, Cisca Wijmenga, Joost HA Martens, Ramnik J Xavier, Colin Logie, Mihai G Netea, Hendrik G Stunnenberg
Publication date
2014/9/26
Journal
science
Volume
345
Issue
6204
Pages
1251086
Publisher
American Association for the Advancement of Science
Description
Introduction
Monocytes circulate in the bloodstream for up to 3 to 5 days. Concomitantly, immunological imprinting of either tolerance (immunosuppression) or trained immunity (innate immune memory) determines the functional fate of monocytes and monocyte-derived macrophages, as observed after infection or vaccination.
The epigenome, DNase I accessibility, and transcriptome were characterized in purified human circulating monocytes, in vitro differentiated naïve, tolerized (immunosuppression), and trained macrophages (innate immune memory). This allowed the identification of pathways functionally implicated in innate immune memory. This epigenetic signature of human monocyte-to-macrophage differentiation and monocyte training generates hypotheses to understand and manipulate medically relevant immune conditions.
Methods
Purified circulating monocytes from healthy volunteers were …
Total citations
Scholar articles
S Saeed, J Quintin, HHD Kerstens, NA Rao… - science, 2014