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While colleges may reflect sexual prejudices of the societal culture at large, they also have the capacity to resist or even transform these troubling viewpoints. Based on longitudinal data collected from 13,881 students nationwide, this study investigates the impact of college experiences on sexually prejudicial attitudes. Results indicate that individuals are more accepting of lesbian, gay, and bisexual relationships after four years of college and that experiential components of racial diversity in the college environment are associated with a decrease in sexual prejudice. Interpretations of findings include the possibility that the observed trends are indicative of sexual prejudice becoming less socially acceptable, as more insidious forms of heterosexism maintain the dominance of the privileged group.