Authors
Julia P Swiercz, Tamiza Nanji, Melanie Gloyd, Alba Guarne, Marie A Elliot
Publication date
2013/4/1
Journal
Nucleic Acids Research
Volume
41
Issue
7
Pages
4171-4184
Publisher
Oxford University Press
Description
Effective chromosome organization is central to the functioning of any cell. In bacteria, this organization is achieved through the concerted activity of multiple nucleoid-associated proteins. These proteins are not, however, universally conserved, and different groups of bacteria have distinct subsets that contribute to chromosome architecture. Here, we describe the characterization of a novel actinobacterial-specific protein in Streptomyces coelicolor. We show that sIHF (SCO1480) associates with the nucleoid and makes important contributions to chromosome condensation and chromosome segregation during Streptomyces sporulation. It also affects antibiotic production, suggesting an additional role in gene regulation. In vitro, sIHF binds DNA in a length-dependent but sequence-independent manner, without any obvious structural preferences. It does, however, impact the activity of topoisomerase, significantly …
Total citations
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Scholar articles
JP Swiercz, T Nanji, M Gloyd, A Guarne, MA Elliot - Nucleic Acids Research, 2013