Authors
Marie A Elliot, Maureen J Bibb, Mark J Buttner, Brenda K Leskiw
Publication date
2001/4
Journal
Molecular microbiology
Volume
40
Issue
1
Pages
257-269
Publisher
Blackwell Science, Ltd
Description
BldD is a transcription factor required for aerial hyphae formation in the filamentous bacterium Streptomyces coelicolor. Three targets of BldD regulation were discovered by a number of means, including examination of bld gene interdependence, selective enrichment of chromosomal DNA fragments bound by BldD and searching the promoter regions of known developmental genes for matches to a previously characterized BldD binding site. The three BldD targets identified were the developmental sigma factor genes, whiG and bldN, and a previously uncharacterized gene, designated bdtA, encoding a putative transcription factor. In each target gene, the sequences bound by BldD were characterized by electrophoretic mobility shift and DNase I footprinting assays, and their alignment suggested AGTgA (n)m TCACc as a consensus BldD operator. The in vivo effect of mutation in bldD on the expression of these …
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Scholar articles
MA Elliot, MJ Bibb, MJ Buttner, BK Leskiw - Molecular microbiology, 2001