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The purpose of this study is to develop a portable capillary electrophoresis (CE) "lab-on-a-chip" device that is integrated with on-chip separation and electrochemical detection (ECD) electrodes. Platinum electrodes were photolithographically patterned on soda lime glass substrates and 20 /spl mu/m by 80 /spl mu/m channels were constructed within a second soda lime glass substrate. To fully realize the portability offered by this microfabricated device, a handheld battery powered CE/ECD circuit was also developed. Experimental flow visualization and computer simulation studies were performed to investigate electrokinetic flow within the microfabricated capillaries. CE/ECD "lab-on-a-chip" devices were successfully fabricated. These devices produced identical electrochemical responses for dopamine and catechol with both the portable electronics and conventional bench top power supply and detection …