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Mate value is a construct of importance to many psychological (especially evolutionary psychological) theories. However, there are no well validated self-report measures of mate value. In light of this, we designed the Mate Value Scale (MVS): a brief, four-item measure that can assess mate value of the self, of one’s partner, or of another target. Study One demonstrates that the MVS has good internal consistency, a one-factor structure, and good convergent validity. Study Two replicates these psychometric findings along with demonstrations of good test–retest reliability and further convergent and discriminant validity. Study Three demonstrates how the MVS can successfully be applied to an evolutionary psychological domain (the sex difference in jealousy). Additional cited research demonstrates the MVS’s utility in a number of other domains. We conclude that the MVS is a short and effective measure of mate value.