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A novel way to estimate the live fuel moisture content (LFMC) was explored from the ratio of canopy water content (CWC) and foliage dry biomass (FDB). The CWC was estimated using the PROSAIL (PROSPECT + SAIL) radiative transfer model from the Landsat 8 product. A weak constraint 4-D variational data assimilation method was employed to assimilate the temporally estimated leaf area index into a soil-water-atmosphere-plant (SWAP) model for optimizing the model control variables. Then, the SWAP model was reinitialized with this optimum set of control variables, and better prediction of FDB was obtained. Results showed that a high accuracy level was achieved for the estimated CWC (R ² = 0.91, RMSE = 84.74 g/m ² ) and FDB (R ² = 0.88, RMSE = 48.54 g/m ² ) when compared with in situ measured values. However, the accuracy level of estimated LFMC was poor (R ² = 0.59, RMSE = 30.85%). Further …