Authors
Gary Williamson, Paul C Engel
Publication date
1982/9/7
Journal
Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology
Volume
706
Issue
2
Pages
245-248
Publisher
Elsevier
Description
The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.3.99.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.
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Scholar articles
G Williamson, PC Engel - Biochimica et Biophysica Acta (BBA)-Protein Structure …, 1982