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Computational Fluid Dynamics (CFD) has become one of the most important tools for the assessment of natural cross-ventilation of buildings. To ensure the accuracy and reliability of CFD simulations, solution verification and validation studies are needed, as well as detailed sensitivity studies to analyse the impact of computational parameters on the results. In a previous study by the present authors, the impact of a wide range of computational parameters on the cross-ventilation flow in a generic isolated single-zone building was investigated. This paper presents the follow-up study that focuses in more detail on validation with wind tunnel measurements and on the effects of physical and numerical diffusion on the cross-ventilation flow. The CFD simulations are performed with the 3D steady Reynolds-Averaged Navier–Stokes (RANS) approach with the SST k–ω model to provide closure. Validation of the coupled …