Authors
Matthew A Ciorba, Terrence E Riehl, M Suprada Rao, Clara Moon, Xueping Ee, Gerardo M Nava, Monica R Walker, Jeffrey M Marinshaw, Thaddeus S Stappenbeck, William F Stenson
Publication date
2012/6/1
Journal
Gut
Volume
61
Issue
6
Pages
829-838
Publisher
BMJ Publishing Group
Description
Background
The small intestinal epithelium is highly sensitive to radiation and is a major site of injury during radiation therapy and environmental overexposure.
Objective
To examine probiotic bacteria as potential radioprotective agents in the intestine.
Methods
8-week-old C57BL/6 wild-type or knockout mice were administered probiotic by gavage for 3 days before 12 Gy whole body radiation. The intestine was evaluated for cell-positional apoptosis (6 h) and crypt survival (84 h).
Results
Gavage of 5×107 Lactobacillus rhamnosus GG (LGG) improved crypt survival about twofold (p<0.01); the effect was observed when administered before, but not after, radiation. Conditioned medium (CM) from LGG improved crypt survival (1.95-fold, p<0.01), and both LGG and LGG-CM reduced epithelial apoptosis particularly at the crypt base (33% to 18%, p<0.01). LGG was detected in the distal ileal contents after the gavage …
Total citations
Scholar articles
MA Ciorba, TE Riehl, MS Rao, C Moon, X Ee, GM Nava… - Gut, 2012