Authors
Terrence E Riehl, David Alvarado, Xueping Ee, Aaron Zuckerman, Lynn Foster, Vaishali Kapoor, Dinesh Thotala, Matthew A Ciorba, William F Stenson
Publication date
2019/6/1
Journal
Gut
Volume
68
Issue
6
Pages
1003-1013
Publisher
BMJ Publishing Group
Description
Objective
Lactobacillus rhamnosus GG (LGG), a probiotic, given by gavage is radioprotective of the mouse intestine. LGG-induced radioprotection is toll-like receptor 2 (TLR2) and cyclooxygenase-2 (COX-2)-dependent and is associated with the migration of COX-2+mesenchymal stem cells (MSCs) from the lamina propria of the villus to the lamina propria near the crypt epithelial stem cells. Our goals were to define the mechanism of LGG radioprotection including identification of the TLR2 agonist, and the mechanism of the MSC migration and to determine the safety and efficacy of this approach in models relevant to clinical radiation therapy.
Design
Intestinal radioprotection was modelled in vitro with cell lines and enteroids as well as in vivo by assaying clinical outcomes and crypt survival. Fractionated abdominal and single dose radiation were used along with syngeneic CT26 colon tumour grafts to assess tumour …
Scholar articles
TE Riehl, D Alvarado, X Ee, A Zuckerman, L Foster… - Gut, 2019