Authors
Gaetana Lanzi, Joachim R De Miranda, Maria Beatrice Boniotti, Craig E Cameron, Antonio Lavazza, Lorenzo Capucci, Scott M Camazine, Cesare Rossi
Publication date
2006/5/15
Journal
Journal of virology
Volume
80
Issue
10
Pages
4998-5009
Publisher
American Society for Microbiology
Description
Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140 nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The coding sequence is flanked by a 1,144-nucleotide 5′ nontranslated leader sequence and a 317-nucleotide 3′ nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32 kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin …
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Scholar articles
G Lanzi, JR De Miranda, MB Boniotti, CE Cameron… - Journal of virology, 2006