Authors
Johannes Gojo, Felix Schmitt-Hoffner, Monika Mauermann, Katja von Hoff, Martin Sill, Andrey Korshunov, Damian Stichel, David Capper, Arnault Tauziede-Espariat, Pascale Varlet, Kenneth Aldape, Zied Abdullaev, Andrew Donson, Jens Pahnke, Ulrich Schüller, Ivy Tran, Kristyn Galbraith, Matija Snuderl, Sanda Alexandrescu, Sebastian Brandner, Maria Łastowska, Evelina Miele, Jasper v Lugt, Lisethe Meijer, Jens Bunt, Christof Kramm, Jordan R Hansford, Lenka Krskova, Michal Zapotocky, Sumihito Nobusawa, David Solomon, Christine Haberler, Barbara Jones, Dominik Sturm, Felix Sahm, Natalie Jäger, Stefan M Pfister, Marcel Kool
Publication date
2022/6/1
Journal
Neuro-Oncology
Volume
24
Issue
Supplement_1
Pages
i50-i50
Publisher
Oxford University Press
Description
BACKGROUND: Embryonal tumors with multilayered rosettes (ETMR) are pediatric brain tumors bearing a grim prognosis, despite intensive multimodal therapeutic approaches. Insights into cellular heterogeneity and cellular communication of tumor cells with cells of the tumor microenvironment (TME), by applying single-cell (sc) techniques, potentially identify mechanisms of therapy resistance and target-directed treatment approaches. MATERIAL AND METHODS: To explore ETMR cell diversity, we used single-cell RNA sequencing (scRNA-seq) in human (n=2) and murine ETMR (transgenic mode; n=4) samples, spatial transcriptomics, 2D and 3D cultures (including co-cultures with TME cells), multiplex immunohistochemistry and drug screens. RESULTS: ETMR microenvironment is composed of tumor and non-tumor cell types. The ETMR malignant compartment harbour cells representing distinct …
Total citations
20242
Scholar articles
J Gojo, F Schmitt-Hoffner, M Mauermann, K von Hoff… - Neuro-Oncology, 2022