Authors
Galatea Kallergi, Vasileia Tsintari, Stelios Sfakianakis, Ekaterini Bei, Eleni Lagoudaki, Anastasios Koutsopoulos, Nefeli Zacharopoulou, Saad Alkahtani, Saud Alarifi, Christos Stournaras, Michalis Zervakis, Vassilis Georgoulias
Publication date
2019/12
Journal
Breast Cancer Research
Volume
21
Pages
1-13
Publisher
BioMed Central
Description
Background
Circulating tumor cells (CTCs) are important for metastatic dissemination of cancer. They can provide useful information, regarding biological features and tumor heterogeneity; however, their detection and characterization are difficult due to their limited number in the bloodstream and their mesenchymal characteristics. Therefore, new biomarkers are needed to address these questions.
Methods
Bioinformatics functional enrichment analysis revealed a subgroup of 24 genes, potentially overexpressed in CTCs. Among these genes, the chemokine receptor CXCR4 plays a central role. After prioritization according to the CXCR4 corresponding pathways, five molecules (JUNB, YWHAB, TYROBP, NFYA, and PRDX1) were selected for further analysis in biological samples. The SKBR3, MDA-MB231, and MCF7 cell lines, as well as PBMCs from …
Scholar articles
G Kallergi, V Tsintari, S Sfakianakis, E Bei… - Breast Cancer Research, 2019