Authors
Yinghao Cao, Yexuan Lin, Na Sun, Xinyi Du, Yixin Dong, Song Mei, Xingyu Deng, Xiaobei Li, Shaoting Guo, Kegong Tang, Jiaqi Liu, Xiangyu Qiao, Dandan Zhao, Yuhao Qin, Cong Zhang, Tianyi Xin, Xiaohu Shi, Congzhao Zhou, Tao Dong, De-an Guo, Benedikt M Kessler, Dong Xu, Jingyuan Song, Fengming Huang, Xiaoyue Wang, Chengyu Jiang
Publication date
2023/10
Journal
Science China Life Sciences
Volume
66
Issue
10
Pages
2380-2398
Publisher
Science China Press
Description
Cross-kingdom herbal miRNA was first reported in 2012. Using a modified herbal extraction protocol, we obtained 73,677,287 sequences by RNA-seq from 245 traditional Chinese Medicine (TCM), of which 20,758,257 were unique sequences. We constructed a Bencao (herbal) small RNA (sRNA) Atlas (http://bencao.bmicc.cn), annotated the sequences by sequence-based clustering, and created a nomenclature system for Bencao sRNAs. The profiles of 21,757 miRNAs in the Atlas were highly consistent with those of plant miRNAs in miRBase. Using software tools, our results demonstrated that all human genes might be regulated by sRNAs from the Bencao sRNA Atlas, part of the predicted human target genes were experimentally validated, suggesting that Bencao sRNAs might be one of the main bioactive components of herbal medicines. We established roadmaps for oligonucleotide drugs development and …
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Scholar articles
Y Cao, Y Lin, N Sun, X Du, Y Dong, S Mei, X Deng, X Li… - Science China Life Sciences, 2023