Authors
Mitchell G Lawrence, Naira V Margaryan, Daniela Loessner, Angus Collins, Kris M Kerr, Megan Turner, Elisabeth A Seftor, Carson R Stephens, John Lai, APC BioResource, Lynne‐Marie Postovit, Judith A Clements, Mary JC Hendrix
Publication date
2011/8/1
Journal
The Prostate
Volume
71
Issue
11
Pages
1198-1209
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Description
BACKGROUND
Nodal is a member of the transforming growth factor β (TGFβ) superfamily that directs embryonic patterning and promotes the plasticity and tumorigenicity of tumor cells, but its role in the prostate is unknown. The goal of this study was to characterize the expression and function of Nodal in prostate cancer and determine whether, like other TGFβ ligands, it modulates androgen receptor (AR) activity.
METHODS
Nodal expression was investigated using immunohistochemistry of tissue microarrays and Western blots of prostate cell lines. The functional role of Nodal was examined using Matrigel and soft agar growth assays. Cross‐talk between Nodal and AR signaling was assessed with luciferase reporter assays and expression of endogenous androgen regulated genes.
RESULTS
Significantly increased Nodal expression was observed in cancer compared with benign prostate specimens. Nodal …
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Scholar articles
MG Lawrence, NV Margaryan, D Loessner, A Collins… - The Prostate, 2011